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Introducción al diseño “in silico” de primers.
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SOLVED: Use the Wallace Rule equation to calculate the Tm of each of the primers you designed. Tm = 2(A+T) + 4(G+C) Left primer: 5' CCTGGACACTGTAC 3' Right primer: 5' AATGTATGAACCTG 3
Solved According to the TM, formula for calculating the | Chegg.com
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Sequences and melting temperature (Tm) of PCR primers used in this study. | Download Table